Journal: Molecular Oncology

Article Title: Tertiary lymphoid structures in head and neck squamous cell carcinoma improve prognosis by recruiting CD8 + T cells

doi: 10.1002/1878-0261.13403

Figure Lengend Snippet: The antitumour effect and pre‐TLS development of Ltα group were blocked by CD8 + cells elimination. (A) Frequency of CD3 + CD8 + T cells among tumour infiltrated lymphocytes from control group mice ( n = 4) and Ltα group mice ( n = 4). The error bars represent the SD of the mean. The asterisks indicate the P ‐value: *< 0.05 (Student's t ‐test). (B) General views of the tongue of the mouse model after CD8 + cells elimination (scale bar: 10 mm) (left). The tumour volume was compared in control group ( n = 5) and Ltα group ( n = 5) (right). The error bars represent the SD of the mean. The P ‐value was obtained using the Student's t ‐test. (C) CD3 + , CD19 + cells and PNAd + vessels in control group ( n = 4) and Ltα group ( n = 4) on the 12th day after CD8 + cells elimination. The error bars represent the SD of the mean. The P ‐values was obtained using the Student's t ‐test. (D) Representative pictures of multiple immunochemical staining of PNAd, CD3 and CD19 in control group ( n = 4) and Ltα group ( n = 4) on the 12th day after CD8 + cells elimination (scale bar: 100 μm).

Article Snippet: The mouse TILs were isolated from the tumours using the tumour lymphocyte infiltration kit (Solarbio, Beijing, China), stained with APC‐conjugated anti‐CD3, PE‐conjugated anti‐CD8 and PerCP‐conjugated anti‐CD4 (Biolegend, San Diego, CA, USA), and detected using flow cytometry.

Techniques: Control, Staining

Journal: Cell reports

Article Title: High Frequency of Shared Clonotypes in Human T Cell Receptor Repertoires

doi: 10.1016/j.celrep.2020.107882

Figure Lengend Snippet: (A) Pairwise percentage clonotype overlaps for naive or memory CD4+ T cell subsets. (B) Pairwise percentage clonotype overlaps for naive or memory CD8+ T cell subsets. See also and and .

Article Snippet: CD4+ Central Memory T Cell Isolation Kit, Human , Miltenyi Biotec , Cat# 130–094-302.

Techniques:

Journal: Cell reports

Article Title: High Frequency of Shared Clonotypes in Human T Cell Receptor Repertoires

doi: 10.1016/j.celrep.2020.107882

Figure Lengend Snippet: The total number of shared clonotypes in each T cell subset belonging to subjects HIP2, HIP3, HIP4, and HIP5 was determined. The percentage overlaps for all five subjects were also determined in each T cell subset and are represented as white text in each figure. (A) CD4+ naive cell subset. (B) CD4+ memory cell subset. (C) CD8+ naive subset. (D) CD8+ memory cell subset. See also .

Article Snippet: CD4+ Central Memory T Cell Isolation Kit, Human , Miltenyi Biotec , Cat# 130–094-302.

Techniques:

Journal: Cell reports

Article Title: High Frequency of Shared Clonotypes in Human T Cell Receptor Repertoires

doi: 10.1016/j.celrep.2020.107882

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD4+ Central Memory T Cell Isolation Kit, Human , Miltenyi Biotec , Cat# 130–094-302.

Techniques: Cell Isolation, Sequencing, Library Quantification, Recombinant, Immunopeptidomics, Western Blot, Software